Regions of Interest may be used to represent coding portions of the DNA, exons/introns, promoters, etc. A region of interest can be defined by selecting the part of the construct that will correspond to the region and then choosing Make Region from the Construct Menu. Overlapping regions can be defined by choosing appropriate segments on the DNA. If no DNA is currently selected, you can still define a new Region of Interest by choosing the Make Region command from the Display Submenu and then typing in the end points of the new region to be created.
Once a region is formed, it can be selected by clicking once. The appearance of a selected region can be altered using the Format Menu – direction, color, and line thickness can be adjusted. Note that changing a line direction for a protein coding region also changes the coding strand on the DNA. Regions can also be rearranged by using move to inside or outside (Display Submenu).
Information can be obtained for any selected region by selecting Get Info… from the Construct Menu. It will show you a dialog box. You may type up to 32,000 characters of comments into each Get Info dialog box. These comments can be searched for key words using the Search Comments… menu option under the Construct Menu. If you check the Protein Sequence button, the amino acid sequence of the region will be displayed when the construct is viewed as a sequence instead of graphically.
Double-clicking a Region of Interest will select the corresponding DNA segment. This is useful for selecting segments of DNA such as polycloning segments, control regions, or origins of replication.
